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The mission of the European Society of Radiology is to serve the healthcare needs of the general public by supporting scientific research, education and training while constantly striving to improve the quality of radiological practice. More Info about ESR. Please browse through our FAQs Frequently Asked Questions or use our page's search function to see if your query has already been answered. For a general contact to the ESR, please click here. Play background video Turn sound on.

ECR Programme Online. Meet your programme planning team. These acts are open to feedback from the public until the 2 February Click here for the ESR feedback on a Proposal for a Regulation on serious cross-border threats to health. Transcription factors often work collectively to achieve this task — scientists speak of cooperativity. While this process is critical for the definition of a cell type during development, the underlying mechanisms for these collective actions are mostly unknown.

Given that co-occupancy of transcription factors is hypothesised to be an important driver of their biological functions, we set out to overcome this limitation. The new approach by Krebs and his team members, known as single-molecule footprinting, can resolve the binding of multiple transcription factors on individual DNA molecules. This allows the researchers to determine if and where transcription factors co-bind their target regions.

They discovered that the degree of co-occupancy is very variable throughout the genome, and mostly independent of which two factors are involved in a pair. Strikingly, co-occupancy of transcription factors scales with how densely a regulatory element is packaged in chromatin. The resolution at which Krebs and his group measured transcription factor and nucleosome binding events is new to the field, making the study the first in which the co-occupancy of transcription factors has been measured at the single-molecule level genome-wide.

While the study was able to answer some longstanding questions about transcription factor cooperativity, it also raised new ones. For instance, mathematical modelling of the co-occupancy data surprisingly revealed that the phenomenon they observed cannot simply be explained by an equilibrium between the binding of transcription factors and histones, as previously proposed.

Their data suggests that recruitment of enzymes to remodel chromatin is likely to be involved in the process.

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